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windows 2011 | PeakMaster 5.3 Release 2011 (portable) /sites/all/res/downloader/locked.png
The program is packed in a zip file. Simply unzip and run PeakMaster.exe
pdf 2014 | PeakMaster 5.3 - Tutorial /sites/all/res/downloader/unlocked.png
A tutorial, which will guide you through the PeakMaster step by step. You will learn how to use PeakMaster on practically-driven examples. The tutorial is accompanied by demo data below.
data_package 2014 | Demo data for PeakMaster tutorial /sites/all/res/downloader/locked.png
We placed the data for PM Tutorial into a separate file in order to make the .pdf file directly accessible in your web browser.
peakmaster 2016 | The most up-to-date PeakMaster database /sites/all/res/downloader/locked.png
We do not update the PeakMaster database very often, since only high quality data are accepted. User database entries are always possible. Anyway, occasionally the database may be updated. Simply replace the Peakmaster.dbc file in your PeakMaster directory. User entries in your original database will be lost. Merging databases is not implemented in PeakMaster.
peakmaster 2016 | PeakMaster Complex /sites/all/res/downloader/notification.png
Looking for PeakMaster Complex? Unfortunately, the software is still under development. It is of limited use only and is not ready for general public. If interested, feel free to contact us.

What is PeakMaster?

PeakMaster is a freeware program useful for people engaged in capillary zone electrophoresis. It predicts parameters of background electrolytes and analyte peaks.

How does PeakMaster work?

First you input the composition of the background electrolyte containing any number of constituents, which can be weak or strong acids or bases or amphoteric electrolytes like aminoacids or peptides. The maximum valence (either positive or negative) is four. The resulting BGE is allowed to be highly acidic or alkaline (usable pH range from 0 to 14).

Optionally you input the names of the analyte ions in the injected sample and some experimental characteristics of your equipment. PeakMaster includes a database based on Takeshi Hirokawa's tables with the data of many ions. PeakMaster further uses Onsager-Fuoss correction of mobilities for ionic strength and Debye-Hückel calculations of activity coefficients.

After clicking the Calculate button, PeakMaster performs the following actions:

  • It calculates BGE characteristics: pH, ionic strength, conductivity, buffer capacity, or concentrations of the individual ionic forms of BGE constituents,
  • It calculates electrophoretic characteristics of the analytes: effective mobility, dispersion tendency, signals in direct detection or conductivity detection or indirect UV detection
  • It simulates electropherograms of a given mixture of analytes
  • It calculates system eigenmobilities of the BGE. The system eigenmobilities are a problematic feature of the BGEs. The existence of system eigenmobilities indicates that certain entities in the electrophoretic system move with such mobilities in the driving electric field. Generally, none of the system eigenmobilities is exactly zero. At least one of the eigenmobilities is almost always very close to zero and is a cause for the injection zone (or water dip, or water gap). It must be, however, realized that the position of the injection zone is not equivalent to a position of an EOF neutral marker and in some BGEs it can even be substantially different. In such BGEs there is no injection zone that can serve as the marker of the EOF! In rather acidic or alkaline BGEs or in BGEs containing multiple coions or in BGEs with a multivalent co-constituent there are one or more eigenmobilities different from zero. System eigenmobilities cause either (i) the appearance of system peaks (system eigenpeaks) moving with the velocity corresponding to the eigenmobility, or (ii) the resonance phenomenon. Due to the resonance, an amplified response of indirect UV detection or conductivity detection at the site of the analyte should be expected. The indirect signal of the analyte is then no longer proportional to its quantity. Further, serious anomalous dispersion of the peak should be expected. We have published a series of papers dealing with these interesting phenomena (Stedry M, Jaros M, Gas B: J. Chromatogr. A 2002, 960, 187-198; Stedry M, Jaros M, Vcelakova K, Gas B: Electrophoresis 2003, 24, 536-547; Stedry, M, Jaros, M, Hruska, V, Gas, B: Electrophoresis 2004, 25, 3071-3079; Jaros M, Hruska V, Stedry M, Zuskova I, Gas B: Electrophoresis 2004, 25, 3080-3085).


PeakMaster allows

  • Optimization of the composition of background electrolyte to give enhanced detector sensitivity and sufficient resolution of analytes while still maintaining acceptable dispersive properties.


Features of PeakMaster

  • Computing algorithm is based on calculation of eigenvalues of a matrix tied to the linearized continuity equations.
  • The BGE can contain any number of constituents
  • The constituents of the BGE and analytes can be weak or strong acids or bases or amphoteric electrolytes
  • pH and buffer capacity of the BGE is calculated
  • Detection signals can be displayed as electropherograms
  • For depiction of electropherograms we utilize Haarhoff-van der Linde (HVL) function, so the peaks look close to reality.